Local resolution of maps from Relion

Tips
1

Hi -
I was wondering if there is a way in the latest version to import half maps from Relion and just run a local resolution map analysis in CryoSparc. I see that there is the option “half map A” and “b” in CryoSparc when importing 3D volume, but then, I can’t make the local resolution work on them. Has anyone an idea of the best way to do it without having to re-run a 3D refinement in CryoSparc?

Thanks,
Best,
Nick

2

AFAIK, by default Cryosparc uses blocres from bsoft (which you can download and use outside the cryosparc system).

3

ok, thanks a lot for the reply

4

Hi @ncoudray, @peter.cherepanov,

In fact cryoSPARC does not use blocres, it’s our own implementation with modifications (also GPU accelerated).
To use external half-maps, you need to import both half-maps separately, and then use the “Low Level Results” system. There is a tutorial for exactly this case here:
https://cryosparc.com/docs/tutorials/job-builder/#use-case-local-resolution-estimation

Hope this helps!

5

Thank you so much for the info!