Hello Everyone,
I am working with a protein with 2 dynamic regions. To achieve high resolution I have subtracted one domain and performed local refinement on the other. I did the same for the other domains as well. I am trying to crop the box to further push resolution of each domain. However, cropping the box results the map of the domain not aligning with the map of the complete protein.
My objective is to have high resolution maps of both the domains in a smaller box size that aligns with the map of the complete protein.
The other option is to use Align 3D maps and align the cropped maps of each domain to the complete protein. Should I move the mask and half maps as well for validation and is this a valid approach? The only concern I have is if there would be any further issues that I am unaware of during map submission.
Thanks