@MmM Please can you
- post error tracebacks as text to make it easier for other forum user to search for and find relevant forum topics.
- post the the CryoSPARC version
- if your CryoSPARC version is v5.0.4 or below, check whether the error persists after updating CryoSPARC to the latest version (see Error during local refinement: "object has no attribute 'seek'" - #13 by mmclean)