Hi, trying the homogeneous ab-initio refinement for the first time. As per the guide, I connected the two half maps to a local refinement job. I am now struggling with this error. Any thoughts?
@MmM Please can you
- post error tracebacks as text to make it easier for other forum user to search for and find relevant forum topics.
- post the the CryoSPARC version
- if your CryoSPARC version is v5.0.4 or below, check whether the error persists after updating CryoSPARC to the latest version (see Error during local refinement: "object has no attribute 'seek'" - #13 by mmclean)
Hi, here you go:
Traceback (most recent call last):
File "cli/run.py", line 106, in cli.run.run_job
File "cli/run.py", line 211, in cli.run.run_job_function
File "compute/jobs/local_refine/run_localrefine.py", line 1240, in compute.jobs.local_refine.run_localrefine.run_local_refine
File "/opt/cryosparc_worker/core/run_context.py", line 198, in log_plot
return jobs.add_event_log_figure(
^^^^^^^^^^^^^^^^^^^^^^^^^^
File "/opt/cryosparc_worker/core/jobs.py", line 2494, in add_event_log_figure
imgfiles = assets.upload_figure_formats(
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "/opt/cryosparc_worker/core/assets.py", line 167, in upload_figure_formats
upload_figure(figure, project_uid, job_or_session_uid, name, format=format, savefig_kw=savefig_kw)
File "/opt/cryosparc_worker/core/assets.py", line 144, in upload_figure
file.seek(0, 0) # type: ignore
^^^^^^^^^
AttributeError: 'NoneType' object has no attribute 'seek'
- v 5.0.2
- Thanks, I have to ask our system admin to do that. Initially, I was looking for ab-initio related errors, not for local refinement ones.
Did you observe any errors during ab initio 3D reconstruction?
Hey, updating to the latest CS version solved the issue. Thanks!
1 Like
@mhmd2 What is your CryoSPARC version? Please let us know if the error persists in the latest version of CryoSPARC.
CryoSPARC V5.0.4
yes, the error still not go.
A fix for this (or a similar) issue was released after CryoSPARC v5.0.4. Please let us know if the error persists after updating CryoSPARC to the most recent version (v5.0.7 as of August 2026).