Local motion correction

Motion Correction
1

Hi All,
I keep getting this error message for the motion correction.
I have no idea on what to do?

Launching job on lane default target vega-irb-usi-ch …

License is valid.

Running job on master node hostname vega-irb-usi-ch

Project P12 Job J84 Started

Master running v2.5.0, worker running v2.5.0

Traceback (most recent call last): File “cryosparc2_worker/cryosparc2_compute/run.py”, line 49, in cryosparc2_compute.run.main File “cryosparc2_compute/jobs/runcommon.py”, line 325, in check_default_inputs assert False, 'Non-optional inputs from the following input groups and their slots are not connected: ’ + missing_inputs + ‘. Please connect all required inputs.’ AssertionError: Non-optional inputs from the following input groups and their slots are not connected: movies.movie_blob, movies.background_blob, movies.rigid_motion. Please connect all required inputs.

2

Hi @Laurent,

From what I’ve gathered you’re trying to run the Local Motion Correction job. Have you connected all required inputs including movies, micrographs and their particles? Take a look at this post to ensure you’re using the job builder correctly: https://cryosparc.com/docs/tutorials/job-builder/#building-jobs-with-input-and-output-groups
Otherwise, if these are movies that have been already motion corrected by the MotionCor2 wrapper, you can simply use the “Extract from Micrographs” job to extract the particle blobs.

3

Thank you very much Sarulthasan.
I look into it and see if it works.
Thanks.
Laurent

4

Hi Stephan,
I understand the problem now.
in fact I do not have a movie, so I used the micrograph import. As a result, I do not have micrograph_blob.
But I guess it is not point do correct motion when you only micrograph.
Thank you again.
Laurent