When I try to run a local motion correction job with my full data set (2422 micrographs), my job eventually fails. It is never in the same exact spot, but it is usually around 850 micrographs, 4000 seconds. If I run jobs of <800 micrographs, the job can finish. Any ideas of how I can get the job to finish with the full set. I can’t see from the output or logs what the issue might be:
Job Overview, follow latest
Processed 850 of 2422 movies in 4772.71s
(864 of 2422) Processing J104/imported/Aug09_07.47.11.mrc
Motion correcting and extracting 9 particles (9 rejected near edges)
Loading raw movie data from J104/imported/Aug09_07.47.11.mrc ...Done in 1.88s
Processing ...Done in 1.37s
Curvature 1.52581e+14 Smooth lambda cal 20.0
Writing out particles.. Done in 0.06s
(865 of 2422) Processing J104/imported/Aug09_07.48.27.mrc
Motion correcting and extracting 80 particles (13 rejected near edges)
Loading raw movie data from J104/imported/Aug09_07.48.27.mrc ...Done in 1.89s
Processing ...Done in 2.22s
Curvature 7.07935e+13 Smooth lambda cal 20.0
Writing out particles.. Done in 0.45s
(866 of 2422) Processing J104/imported/Aug09_07.49.42.mrc
Motion correcting and extracting 6 particles (6 rejected near edges)
Loading raw movie data from J104/imported/Aug09_07.49.42.mrc ...Done in 1.86s
Processing ...Done in 1.35s
Curvature 8.81582e+13 Smooth lambda cal 20.0
Writing out particles.. Done in 0.04s
(867 of 2422) Processing J104/imported/Aug09_07.50.56.mrc
Motion correcting and extracting 84 particles (16 rejected near edges)
Loading raw movie data from J104/imported/Aug09_07.50.56.mrc ...Done in 1.80s
Processing ...Done in 2.09s
Curvature 8.6355e+13 Smooth lambda cal 20.0
Writing out particles.. Done in 0.50s
(868 of 2422) Processing J104/imported/Aug09_07.52.08.mrc
Motion correcting and extracting 78 particles (17 rejected near edges)
Loading raw movie data from J104/imported/Aug09_07.52.08.mrc ...Done in 2.12s
Processing ...Done in 2.19s
Curvature 5.73468e+13 Smooth lambda cal 20.0
Writing out particles.. Done in 1.58s
(869 of 2422) Processing J104/imported/Aug09_07.53.19.mrc
Motion correcting and extracting 42 particles (13 rejected near edges)
Loading raw movie data from J104/imported/Aug09_07.53.19.mrc ...Done in 2.32s
Processing ...Done in 1.78s
Curvature 5.0885e+13 Smooth lambda cal 20.0
Writing out particles.. Done in 0.24s
(870 of 2422) Processing J104/imported/Aug09_07.54.31.mrc
Motion correcting and extracting 81 particles (20 rejected near edges)
Loading raw movie data from J104/imported/Aug09_07.54.31.mrc ...Done in 2.37s
Processing ...Done in 2.17s
Curvature 5.9319e+13 Smooth lambda cal 20.0
Writing out particles.. Done in 1.38s
(871 of 2422) Processing J104/imported/Aug09_07.56.00.mrc
Motion correcting and extracting 96 particles (23 rejected near edges)
Loading raw movie data from J104/imported/Aug09_07.56.00.mrc ...Done in 2.07s
Processing ...Done in 2.24s
Curvature 7.59e+13 Smooth lambda cal 20.0
Writing out particles.. Done in 0.51s
(872 of 2422) Processing J104/imported/Aug09_07.57.08.mrc
Motion correcting and extracting 73 particles (17 rejected near edges)
Loading raw movie data from J104/imported/Aug09_07.57.08.mrc ...Done in 1.75s
Processing ...Done in 1.99s
Curvature 7.31061e+13 Smooth lambda cal 20.0
Writing out particles.. Done in 1.25s
(873 of 2422) Processing J104/imported/Aug09_07.58.29.mrc
Motion correcting and extracting 122 particles (34 rejected near edges)
Loading raw movie data from J104/imported/Aug09_07.58.29.mrc ...Done in 3.81s
Processing ...Done in 2.46s
Curvature 1.0665e+14 Smooth lambda cal 20.0
Writing out particles.. Done in 1.58s
(874 of 2422) Processing J104/imported/Aug09_07.59.45.mrc
Motion correcting and extracting 110 particles (31 rejected near edges)
Loading raw movie data from J104/imported/Aug09_07.59.45.mrc ...Done in 2.43s
Processing ...Done in 2.30s
Curvature 1.24052e+14 Smooth lambda cal 20.0
Writing out particles.. Done in 0.63s
(875 of 2422) Processing J104/imported/Aug09_08.01.00.mrc
Motion correcting and extracting 138 particles (30 rejected near edges)
Loading raw movie data from J104/imported/Aug09_08.01.00.mrc ...Done in 2.19s
Processing ...Done in 2.75s
Curvature 7.24537e+13 Smooth lambda cal 20.0
Writing out particles.. Done in 2.56s
(876 of 2422) Processing J104/imported/Aug09_08.02.15.mrc
Motion correcting and extracting 107 particles (31 rejected near edges)
Loading raw movie data from J104/imported/Aug09_08.02.15.mrc ...Done in 2.59s
Processing ...Done in 2.53s
Curvature 1.47105e+14 Smooth lambda cal 20.0
Writing out particles.. Done in 0.61s
(877 of 2422) Processing J104/imported/Aug09_08.03.31.mrc
Motion correcting and extracting 123 particles (20 rejected near edges)
Loading raw movie data from J104/imported/Aug09_08.03.31.mrc ...Done in 2.55s
Processing ...Done in 2.49s
Curvature 1.17303e+14 Smooth lambda cal 20.0
Writing out particles.. Done in 1.62s
(878 of 2422) Processing J104/imported/Aug09_08.04.59.mrc
Motion correcting and extracting 96 particles (20 rejected near edges)
Loading raw movie data from J104/imported/Aug09_08.04.59.mrc ...Done in 2.73s
Processing ...Done in 2.21s
Curvature 9.37436e+13 Smooth lambda cal 20.0
Writing out particles.. Done in 0.53s
(879 of 2422) Processing J104/imported/Aug09_08.06.11.mrc
Motion correcting and extracting 93 particles (20 rejected near edges)
Loading raw movie data from J104/imported/Aug09_08.06.11.mrc ...Done in 2.94s
Processing ...Done in 2.13s
Curvature 8.61964e+13 Smooth lambda cal 20.0
Writing out particles.. Done in 2.29s
(880 of 2422) Processing J104/imported/Aug09_08.07.22.mrc
Motion correcting and extracting 95 particles (20 rejected near edges)
Loading raw movie data from J104/imported/Aug09_08.07.22.mrc ...Done in 2.35s
Processing ...Done in 2.31s
Curvature 6.33217e+13 Smooth lambda cal 20.0
Writing out particles.. Done in 0.57s
(881 of 2422) Processing J104/imported/Aug09_08.08.37.mrc
Motion correcting and extracting 78 particles (9 rejected near edges)
Loading raw movie data from J104/imported/Aug09_08.08.37.mrc ...Done in 2.21s
Processing ...Done in 2.18s
Curvature 4.57629e+13 Smooth lambda cal 20.0
Writing out particles.. Done in 0.47s
(882 of 2422) Processing J104/imported/Aug09_08.09.49.mrc
Motion correcting and extracting 98 particles (26 rejected near edges)
Loading raw movie data from J104/imported/Aug09_08.09.49.mrc ...Done in 1.68s
Processing ...Done in 2.36s
Curvature 5.67702e+13 Smooth lambda cal 20.0
Writing out particles.. Done in 0.85s
(883 of 2422) Processing J104/imported/Aug09_08.11.15.mrc
Motion correcting and extracting 127 particles (28 rejected near edges)
Loading raw movie data from J104/imported/Aug09_08.11.15.mrc ...Done in 2.10s
Processing ...Done in 2.46s
jobs.log
================= CRYOSPARCW ======= 2019-08-23 16:51:55.329512 =========
Project P2 Job J145
Master m9g-1-20.rc.byu.edu Port 39002
===========================================================================
========= monitor process now starting main process
MAINPROCESS PID 66483
MAIN PID 66483
motioncorrection.run_local cryosparc2_compute.jobs.jobregister
========= monitor process now waiting for main process
/zhome/pianonan/software/cryosparc/cryosparc2_worker/deps/anaconda/lib/python2.7/site-packages/skcuda/cublas.py:283: UserWarning: creating CUBLAS context to get version number
warnings.warn('creating CUBLAS context to get version number')
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/zhome/pianonan/software/cryosparc/cryosparc2_worker/deps/anaconda/lib/python2.7/site-packages/matplotlib/pyplot.py:516: RuntimeWarning: More than 20 figures have been opened. Figures created through the pyplot interface (`matplotlib.pyplot.figure`) are retained until explicitly closed and may consume too much memory. (To control this warning, see the rcParam `figure.max_open_warning`).
max_open_warning, RuntimeWarning)
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...repeated 500 times...
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========= main process now complete.
========= monitor process now complete.
Current cryoSPARC version: v2.9.0
CRYOSPARC_CUDA_PATH
: /apps/cuda/9.2.88
OS: Ubuntu