Interpreting deep in FSC curve after autotightening

[marino-j]

Hi, can someone comment on the FSC obtained, especially the deep around 8 A of the corrected curve ? Many thanks !

[vamsee]

Hi @marino-j

Check this post. Has some nice descriptions on what could be happening.

Tight, corrected and loose GSFSC curves

Hi All, The dip is an artifact of the boundary where phases are randomization and where they are experimental. The same artifact is seen in Relion’s phase randomization for mask correction and extensive discussions about it can be seen in ccpem’s archive. It has been suggested that the ‘dip’ points be removed from the curve to make it look nicer, but I believe it is preferable to leave them in to show that correction has been done. If the corrected FSC is lower than the tight masked FSC then th…

[marino-j]

Many thanks for pointing me to this relevant discussion, which I have missed before posting… many thanks !