Inspect Picks functionality

Dear colleagues,

Could you please add the possibility for manual adding // removing the particles?

For removing it would be nice to have something fast as area eraser.

Thanks in advance

Kind regards,

This functionality may already be available in Manual Picker, which allows inputs of existing particles.

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Dear @wtempel,

Correct me if I am wrong, but the adding/removing button is only present in Interactive Job: Manual Picker.
What I meant is

  1. run the auto-picking (template, topaz, deep learning etc.)
  2. After the auto-picking process, be able to manually add // remove the good//bad particles going through the dataset manually. Namely, adding the picking // removing tool that you already have for Interactive Job: Manual Picker (arrow + and arrow minus)

Both Interactive Jobs: Inspect Particle Picks & Curation exposures can work fine for this aim.

Thank you.

Kind regards,

Please try if the Manual Picker can do this for you already after you connect

  • Micrographs
  • auto-picked Particles

Dear @wtempel and @mmclean great job! everything works as you described.

Just one addition - how to specify/change the radius of the erasing arrow?
I noticed that when 2 particles, good and bad are too close to each other - both are removed.
Can you make the -arrow as a circle and make this erasing arrow circle radius easy changeable?

Thanks in advance.

Kind regards,

Hi @Dmitry ,

Thank you for the suggestion. We have made a note of it and will work to improve this in a future release.

- Suhail

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Dear Suhail @sdawood,

One more suggestion towards the CS improvement in picking -
it would be great to add the manual helix picking tool on top of single particles one (the picking + and - arrows).
Similar to the one relion has (A microtubule RELION-based pipeline for cryo-EM image processing | bioRxiv)

For some of the projects it will be very beneficial.

Kind regards,

Thanks @Dmitry, we’ve noted the request and hope to include a “straight filament” mode in manual picker at some point :slight_smile:


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