I extracted particles from RELION/4.0, and I decided to transfer those particles in CryoSPARC for faster 2D classification clean up. Now, I am trying to send the cleaned particles stack back to RELION/4.0 for further processing.
I used the command below:
csparc2star.py --swapxy /ddn/cryoemCore/process/cryosparc_callisto/Shears/P114/J3144/particles_selected.cs /ddn/cryoemCore/process/cryosparc_callisto/Shears/P114/J3144/P114_J3144_passthrough_particles_selected.cs ~/GZ60_1Flat.star --strip-uid
The star file was successfully created. Unfortunately, the particles path still has the uid for the micrographs. I anticipate that because of this, I won’t be able to re-extract these particles again in Relion/4.0.
000275@J3140/imported/002601468735142943058_20220824_ASP1_GZ60_1_000_0_0.mrcs 24.795918 17.568200 3.02 9000 11499.257812 11359.625977 -85.321602 0.000000 0.000000 81 2
Is my concern valid? If so, how to I address this concern? Thanks!