Import the Topaz COVID-19 spike picking model to use in your projects

Cryo-EM Data Processing
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Shared by Alex Noble on Twitter here: https://twitter.com/alexjamesnoble/status/1273670231400034307

You can now download the Topaz picking model used in the manuscript here:

Please cite this manuscript if you use this model.

To import into cryoSPARC,

  1. Download the compressed folder that is linked in the original tweet above
  2. De-compress the folder
    tar -xf topaz_COVID19_picking_model_P257_J75.tar.bz2
  3. Create a new directory inside your project folder named “imports” (if you don’t already have one)
    mkdir -p /bulk/projects/P23/imports
  4. Copy the folder topaz_COVID19_picking_model_P257_J75 into this directory:
    cp -r ./topaz_COVID19_picking_model_P257_J75 /bulk/projects/P23/imports
  5. In cryoSPARC, navigate to your workspace and create the “Import Result Group” job
    Screen Shot 2020-06-18 at 2.22.22 PM
  6. For the Absolute path to .csg file parameter, specify the path to the .csg file inside the topaz model folder:
    /bulk/projects/P23/imports/topaz_COVID19_picking_model_P257_J75/P257_J75_topaz_model/P257_J75_topaz_model_exported.csg
  7. Queue the job, and the Topaz model will be available to use as the output of this job

Happy processing!

1 Like
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I see a version incompatibility. I have Topaz 0.2.4 installed while the model was created in 0.2.3. Any way I can work around that?