Hi, I did 3D classification of a CryoSPARC created particle set in Relion3 and want to import the selected subset of particles back to CS for further processing. Although in the .star file the particle stack point to the original ones created by CS (in the JXXX folder), CS treats the imported particle stack as an independent one. There are two disadvantages: (1) In the subsequent processing, CS will make another copy of the original particle stack in /scratch (2) the imported stack loses some useful information such as the “scale” for each particle before entering Relion3.
I wonder if I can use the Particle Sets Tool (intersect) in CS to re-connect the imported particle stack to the original ones. This should be possible because they are pointing to the same .mrcs particle files.