the particles have a strong density of the bilayer picked from vesicles, and are difficult to align in 2D classification. I try to mask the bilayer and have a clearer density of target protein, but I don’t know how to pick particles using 3D volume as references. I also want to pick particles using Relion, But I don’t know how to import the micrographs exported from live session of CryoSPARC to Relion.
Do you know how to fix it?
Thank you very much!