Icosahedral Symmetry Orientation

3D Reconstruction
1

Hi Everyone,

I have a question about the icosahedral symmetry orientation. I want to implement icosahedral symmetry such that the 3 fold axis sits on the Z-axis. I’m have tried I, I1, I2, I3, and I5 and cannot seem to orient my density in this orientation. Can someone please advise me on how to proceed with this request? Thank you in advance.

Sean

2

Hi @h.sean!

There is not currently a way to use this symmetry convention in CryoSPARC. Can you share a bit more why you wan to use this convention instead of the more common ones with a 2-fold axis on Z?

3

Hi @rposert,

I have a protein (nano body) that binds at specifically the 3 fold axis. Whenever I try to do symmetry expansion for local refinement, the view of the particle is not ideal. But, This could be a user error in the volume alignment. Having the 3 fold on the Z axis would make life a bit easier in my case to do the refinements with my current workflow. The particles I am working with are relatively flat (think soccer ball).