How to improve local refinement of fibres

Hi all,

I am working with some long fibres from which I can obtain a decent 6 A map, but I am wondering if anyone has any experience or recommendations on how to change the default parameters, specially during local refinement. They are rather flexible but I am able to cluster them efficiently using 3D variability, so heterogeneity seems to be manageable.

I have been changing the “Use pose/shift gaussian prior during alignment” and the subsequent values, but I am doing it a bit blindly. Are there any values that would make the most sense? Is there any other parameter I should be aware of?

Looking forward to any advice!