How to flip map

3D Reconstruction
1

Hi,

I’m getting a reconstruction that has the wrong hand and I couldn’t find the way to flip it.

Thanks in advance for your help!

Best,

Maria

2

Hi Maria,

You should be able to use the Volume Tools job to flip the structure handedness. If you do not set the threshold parameter, and select the input/output types appropriately it will only flip the structure and do nothing else, as you need.

Best,
Ali

3

Hi,
You could flip it on CHIMERA using following command: vop zflip #0
change the number to correspond to the map number on chimera. Then save the flipped 3D map and re-import into cryosparc?

I’m sure there’s a simpler way but this is the method I use.

Best wishes,
Safi

5

If using UCSF ChimeraX and volume of interest is ID 1 (#1), slightly different syntax in ChimeraX

volume flip #1 axis z