Hi @samhaysom,
The map_filtered output in non-uniform refinement is generated as follows, after refinement has converged:
- both raw, unfiltered halfmaps are averaged together
- the raw map is filtered using the Gold-Standard FSC curve
- the filtered map is sharpened using the Guinier-plot estimated b-factor
- the sharpened map is outputted as
_map_sharp_local.mrc(confusing filename… sorry) - the sharpened map is locally filtered using a local resolution estimate computed from the half-maps
- the locally filtered map is outputted as
_map_filtered.mrc
You can replicate these steps by doing:
- compute local resolution using the volume output of the nu-refine job, in a local resolution estimation job (with default parameters)
- create a local filtering job. Using the low-level results interface of the job builder (i.e. expand the input slots to edit individual connections) to connect the
map_filteredoutput from the nu-refine job and themap_locresoutput from the local resolution estimation job - leave all local filtering parameters as default, and run the job