Highpass filter for micrograph visualization?

Particle Picking
1

Hi,

Would it be possible at some point to add an interactive highpass filter (maybe a fixed frequency, e.g. 150Å) to Manual Picker, to complement the existing lowpass filter slider?

In cases of “bumpy” or uneven ice, applying a ~100-200Å highpass filter can be helpful to flatten out the uneven background, making manual picking easier. This can be particularly the case after denoising, where otherwise imperceptible features of the background can become more prominent.

Cheers
Oli

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2

Thanks @olibclarke – this makes good sense and we’ve noted this feedback!

Best,
Michael

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3

Thanks Michael! It would also be great to have a highpass (lower res limit) option in 2D classification - currently one can do this by using the downsample particles tool to generate a highpass filtered set, but it would be useful to have it built in

4

Hi @olibclarke,

Thanks for the request! Highpass filtering in 2D classification should be available in a (not too distant) future version of CryoSPARC.

Best,
Michael

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