I am having a problem with performing heterogenous refinement.
The first iteration is ok (17.3 A and 17.9 A estimated resolution respectively) but after the second iteration (8.92 A and 8.92 A estimated resolution) it doesnt increase the resolution and moreover the FSC curve graph stops at around 9 (see attahec image). I am using the same particles and ab-initio model that works very well for homogeneous refinement reaching 3.8 A so I am puzzled.
I am wondering if anyone else had the same problem?
thanks in advance for any feedback