Hi all,
is there a way of doing a local heterogeneous refinement. As far as I see I can’t ask for several output maps in the local refinement but I also can’t add a mask to the heterogeneous refinement. Is there some workaround.
Best,
David
No, unfortunately not as yet
signal subtraction tho, right?
@CryoEM1 you can’t use a mask in Het. Refine, so the subtraction needs to be perfect for that to work.
well I guess I guess this is then rather a feature request 
Feature request recorded
We are currently reworking local refinement jobs and will extend to allow heterogeneity.
4 Likes
would still love to see this - and with an arbitrarily small search range this should approximate classification without alignment, which is very powerful for separating out local heterogeneity
4 Likes
Hi Ali,
Is this still in the pipeline? Classification without alignments is great, but is not suited for every case - some form of local/masked heterogeneous refinement would still be very useful!
Cheers
Oli
Hi @olibclarke, thanks for following up 
it is still on the roadmap but we don’t have a definite timeline!
3 Likes