HELP! I have trouble in negative stain data processing

[LHW]

1.I do ‘import micrograph’ at first
2. Then I try ‘patch CTF estimation(multi)’, and failed, all of them are incomplete.
I’m a novice here and I don’t know why.Can someone help me?

[wtempel]

Welcome to the forum @LHW.
Did you confirm that your imported data are already “single frame” and would not require motion correction?
Please can you provide additional information about your computer:

@stephan

cryoSPARC worker environment

• CUDA toolkit and pycuda driver information
  eval $(<path-to-cryosparc_worker>/bin/cryosparcw env)
${CRYOSPARC_CUDA_PATH}/bin/nvcc --version
python -c "import pycuda.driver; print(pycuda.driver.get_version())"
• Output of
  uname -a && free -g && nvidia-smi
  on relevant worker node(s)

and the ouput of
cryosparcm joblog P5 J16

[LHW]

Thanks for your answer! My teacher said someone else were using Relion at that time, so the computer was busy and didn’t have extra capacity to run cryoSPARC, that’s why I failed.
My teacher repeated what I had done in few hours later, and all of them were successful. Wow, so amazing.
Finally, thank you again for your reply and have a good day!（Forgive my grammatical mistakes, if any）