Hi, I am hoping this question has a trivial answer. I have a helical refinement job that gives a resolution of 2.4 angstrom resolution. To test the Ewald correction, I ran two homogeneous reconstruction jobs with the two opposite Ewald signs, inputting the particles and mask from the helical refinement jobs.
However, the resolution got much worse in both the reconstruction jobs (3.0 vs 2.9). I see that the per-particle scale factor distribution in the reconstructions is uniformly set to 1, which I suspect could be the cause of this. So my question is:
Could it be that the homogenous reconstruction somehow ignored per-particle scale factors in from my helically refined particles?
A follow-up question would be, if so, how would I fix homogeneous reconstruction to include the scale factors?
Thanks! - Chuck