I think it would be great if some sort of heterogenous refinement for helical filaments could be added in the future. This way particles that don’t align too well to the helical parameters could be pruned out, along with removing junk.
In an attempt to do this I have tried to do ab-initio with aligned filament particles to produce multiple starting models but for some reason none of the maps can be downloaded. I’m not sure if this is a bug or just something that isn’t implemented yet but I just wanted you know.
Thanks and best wishes,