Having issues importing particles from relion

Import and Export
1

I am getting this error when trying to import particles from relion. Any suggestions or workarounds would be amazing.

[CPU: 399.9 MB]  Traceback (most recent call last):
  File "cryosparc_master/cryosparc_compute/run.py", line 85, in cryosparc_compute.run.main
  File "/mnt/ishtar-data/cryosparc2/cryosparc2_master/cryosparc_compute/jobs/imports/run.py", line 452, in run_import_particles
    fig = plotutil.plot_images_simple(dispparts, rows=4, cols=8, radwn=6, figscale=1.5)
  File "/mnt/ishtar-data/cryosparc2/cryosparc2_master/cryosparc_compute/plotutil.py", line 581, in plot_images_simple
    rD = img.get_original_real_data()
  File "/mnt/ishtar-data/cryosparc2/cryosparc2_master/cryosparc_compute/particles.py", line 22, in get_original_real_data
    return self.blob.view().copy()
  File "/mnt/ishtar-data/cryosparc2/cryosparc2_master/cryosparc_compute/blobio/mrc.py", line 127, in view
    return self.get()
  File "/mnt/ishtar-data/cryosparc2/cryosparc2_master/cryosparc_compute/blobio/mrc.py", line 122, in get
    _, data, total_time = prefetch.synchronous_native_read(self.fname, idx_start = self.page, idx_limit = self.page+1)
  File "cryosparc_master/cryosparc_compute/blobio/prefetch.py", line 67, in cryosparc_compute.blobio.prefetch.synchronous_native_read
RuntimeError: dtype_to_bytes 462: Invalid argument
[arguments] /mnt/mawu-data/asmith/cryosparc2/NECwNup/P144/J603/imported/004657894949528296186_20201022_asmith_NECwNup_ssDNA3_261_0003_aligned_mic_DW.mrcs, 0, 0, 4, 5, 2, 40
2

It looks like your particles are in float16 format which is not compatible with cryosparc.

Best,

Ryan

3

@ammariesm float16 support is available through a recent patch for version 3.3.1. Please see the patch announcement for additional details.