I am a beginner regarding the software,
so I would like to know 3 things:
-
does the GSFSC resolution indicate the final resolution of the protein?
-
will doing a “homogeneous refinement” several times make the resolution better?
(if GSFSC does indicate the resolution, and to have tested the resolution seems better, but is it really the case?) -
When it is usefull to extract micrograph ?