Hi, team cryoSPARC,
I am running the latest version of cryoSPARC and trying to process a negatively stained dataset for a relatively small protein (80 kDa). I noticed that the 2D classes during the iteration step appear fine but once the iterations finish, the 2D classes appear fuzzy and streaky. I tried varying the box sizes but still, I observe similar results. I have attached the image of the 2D classes during the iteration and after all the iterations are finished.
Any suggestion is highly appreciated!!!
Thanks.