Hi,
When I run Non-uniform Refinement, the FSC curves are all above the 0.143 threshold. I could tell the map details and resolution varied, but the map resolution kept showing 4.34A. The true resolution of the map is probably around 5A, and there is almost no gradient. I have tried Remove Duplicate Particles, but it didn’t help. Has anyone experienced this before? Is it due to overfitting? What can be done to fix it?
Welcome to the forums! It looks like you are using binned particles - see similar issue here: FSC resolution above 0.143 - #4 by olibclarke
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I tried unbinned particles and the curves looked normal. Thank you!
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