I am working on fibrils and i want to pick filament in the manual picker but i am unable to find it. Is there any option like that or can someone guide me how can i do?? I need filaments to proceed further.
Welcome to the forum @shivamkur . Have you looked at the tutorial?
Yeah I have seen the tutorial about it. Actually the problem is with my data, we have data of protein aggregates where the fibres are looking very heterogeneous, tangled and branched. The fibre length which between two branched section is around 300-400 px. When i run the job then it picks the fibres of bigger length and includes branches which is adding heterogeneity to the data that’s why we are exploring the options for manual filament picking. It would be a great help if you can guide me with it and how can i achieve a homogeneous data.
Hi @shivamkur,
We have noted your feature request to perform manual filament picking!
Would you be bale to post a couple representative micrographs along with the corresponding filament picker diagnostic plots. For the micrographs, it would be good if they were denoised and show picks (or lack thereof) in the images. There might be some combination of Hysteresis thresholds, Radius around crossings to ignore, and Distance to trim from end points parameters that might allow you to fully pick your micrographs without the need for manual picking.
Best,
Kye
Hi @shivamkur,
I am following up on this to see if you would be able to post a few images from your processing?
Best,
Kye
Thankyou Very much for your consideration. I am really sorry that i was not able to reply from a long time, i had some serious medical issues. Can you please guide me, how can i upload the .zip here or i can share you on your email. It has some raw micrographs and the denoised ones, please tell if you need something else.
Best,
Shivam