Feature request: Import tif micrographs

Features and Functionality
1

Hello,

I have a simple request that would result in a lot of saved space: Allow compressed tifs (lzw and zlib) micrographs (ie. frame-aligned images) to be imported into Cryosparc.

Thank you,
-Alex

3 Likes
2

Hi Alex,

Thanks for the suggestion. Do you find that motion-corrected micrographs actually compress well? I would expect that they’d generally be floating-point data, which IME doesn’t take (lossless) compression very well. I’d be interested to know if your experience shows otherwise.

–Harris

3

Hi Harris,

We have a python script to PASR data (frames or frame-aligned micrographs) and save as MRC or tif. For frame-aligned micrographs, there is a significant size difference, and zlib compression is significantly superior for datasets I’ve tried. Overall space saving for this purpose is hundreds of megabytes per micrograph.

PASR reference: Post-acquisition super resolution for cryo-electron microscopy | bioRxiv

Best,
-Alex

2 Likes
4

Hi Alex,

Understood, that makes sense. We’ve recorded this request (though I’m not sure on the exact timeline for releasing it)

Harris