Failed to open file b error during full frame motion correction

Motion Correction
1

Hello, I’m posting a question to get some help.
After I finished the import movie and ran full frame motion correction, the following problem occurred.

Error occurred while processing J5/imported/018113185260254051879_FoilHole_2207185_Data_2182602_2182604_20230204_053332_fractions.tiff
Traceback (most recent call last):
File “/home/cryosparc_user/cryosparc/cryosparc_worker/cryosparc_compute/jobs/pipeline.py”, line 60, in exec
return self.process(item)
File “cryosparc_master/cryosparc_compute/jobs/motioncorrection/run.py”, line 109, in cryosparc_compute.jobs.motioncorrection.run.run_rigid_motion_correction_multi.motionworker.process
File “cryosparc_master/cryosparc_compute/jobs/motioncorrection/run.py”, line 116, in cryosparc_compute.jobs.motioncorrection.run.run_rigid_motion_correction_multi.motionworker.process
File “/home/cryosparc_user/cryosparc/cryosparc_worker/cryosparc_compute/blobio/tiff.py”, line 65, in read_tiff
mov = read_tiff_frames_threaded(path, num_threads)
File “/home/cryosparc_user/cryosparc/cryosparc_worker/cryosparc_compute/blobio/tiff.py”, line 44, in read_tiff_frames_threaded
tif = libtiff.TIFF.open(tifpath, mode=‘r’)
File “/home/cryosparc_user/cryosparc/cryosparc_worker/deps/anaconda/envs/cryosparc_worker_env/lib/python3.8/site-packages/libtiff/libtiff_ctypes.py”, line 503, in open
raise TypeError(‘Failed to open file ’ + repr(filename))
TypeError: Failed to open file b’/home/sbc/nhj/a62_dmaqb1/CS-a62-dmaqb1/J5/imported/018113185260254051879_FoilHole_2207185_Data_2182602_2182604_20230204_053332_fractions.tiff’

Marking J5/imported/018113185260254051879_FoilHole_2207185_Data_2182602_2182604_20230204_053332_fractions.tiff as incomplete and continuing…

I’d be grateful if you could tell me how to solve it.

2

Hi @hjna,

There might be a permissions issue or something of that nature… Have a look in the job log to see if there’s a more detailed error message. You can view the job log from the command line by running “cryosparcm joblog Pxx Jyy” where xx and yy are replaced with the project and job numbers respectively. Or, if you’re running CryoSPARC version 4, you can open up the job in the GUI, go to the metadata tab and then click “Log” to see the same information.

–Harris