Export particles to Relion, invert contrast



Hi all,

I exported particles (from homogenous refinement) to Relion, but it turns out the particles have inverted contrast (black on white). Could anyone please provide any tips to solve this problem?



Hi @ruiruigo,

You may need to use some Relion tools in this case - we are not too familiar with these but perhaps another user on this forum can chime in?


I think relion_image_handler with --multiply_constant -1 should do the trick to invert your particles, but haven’t tried this myself