Hi Dear cryosparc community,
I extracted binned 2 particles in relion and imported them into cryoSparc, did 2D classification and would like to re-extracted the selected particles in relion for futher 3D-reconstrction. I am wondering how to do it correctly: which files do I need to use to create the star file and in relion import, what is the import file option I need to choose (eg. 2D/3D coordinates etc) What I tried so far is:
I used to pyem to combine particles.cs and passthrough_particles.cs file to create a particles_combine.cs file. The output seems all right. But it could not be imported into relion and used to do the extraction.
000004@J10/imported/…_22_04_51.mrcs 234.183655 2.925000 2.275000 300.000000 1$
000005@J10/imported/…22_04_51.mrcs 22.959183 -2.925000 1.625000 300.000000 1$
000006@J10/imported/…22_04_51.mrcs 197.448975 0.975000 -0.975