I have ran a cryosparc live 3.0 session and the motion correction and ctf estimation are completed successfully. I can see the output files (mrc and trajectory) in the corresponding folders. But the particle picking was not ideal and I would like to try out different methods. I understand that we can export the picked particles for further refinement. But I did not find a way to export/import these correct mrcs for further particle picking. I think such functionality is desirable since we don’t have to repeat motion correction and CTF estimation again, that costs time and duplication of space.
Following from the above answer and this one, is there a way to use this export on a different computer?
If we run Live on a facility server during data collection and move motion corrected micrographs to lab workstations/servers for the rest of the processing, we lose motion trajectory and other metadata that could be useful later.
Is there a best practice with Archive or Detach that would allow the exported Live job to be used on a separate machine?
I run Live on the facility computer, copy the entire CS project directory(which only contains my live session) back to the lab server along with my movies, and import the project on the home server. Archiving the project is not required.
Good luck! As you can gather from my post yesterday I’ve run into a little trouble downstream (with reference-based motion correction) after this, I just need to figure out how to solve that in CS5. Say hi to JMB for me.
One should ensure the project directory is in either the archived or, likely preferable given the planned transfer between CryoSPARC instances, the detached state when performing the copy. Failure to do so increases the risk of inconsistent or corrupted data or metadata files.