Error with CryoSPARC live v4.6.2

CryoSPARC Live
1

Hi guys,
I was tring to use cryosparc live 4.6.2 to process images in real time. but it gives errors like this below. Does anyone have any idea what is going on?

Traceback (most recent call last):
  File "cryosparc_master/cryosparc_compute/jobs/rtp_workers/run.py", line 381, in cryosparc_master.cryosparc_compute.jobs.rtp_workers.run.rtp_worker
  File "cryosparc_master/cryosparc_compute/jobs/rtp_workers/run.py", line 450, in cryosparc_master.cryosparc_compute.jobs.rtp_workers.run.process_movie
  File "cryosparc_master/cryosparc_compute/jobs/rtp_workers/run.py", line 514, in cryosparc_master.cryosparc_compute.jobs.rtp_workers.run.do_check
  File "cryosparc_master/cryosparc_compute/jobs/rtp_workers/run.py", line 149, in cryosparc_master.cryosparc_compute.jobs.rtp_workers.run.RTPExposureCache.cache_read_gainref_header
  File "cryosparc_master/cryosparc_compute/jobs/rtp_workers/run.py", line 138, in cryosparc_master.cryosparc_compute.jobs.rtp_workers.run.RTPExposureCache.read_header_sync
  File "cryosparc_master/cryosparc_compute/blobio/prefetch.py", line 41, in cryosparc_master.cryosparc_compute.blobio.prefetch.Prefetch.__init__
  File "cryosparc_master/cryosparc_compute/blobio/prefetch.py", line 119, in cryosparc_master.cryosparc_compute.blobio.prefetch.validate_read_parameters
Exception: Unsupported file extension (must be .mrc, .mrcs, .mrc.bz2, .tif, .eer, .ecc, .gain)
![image|690x65](upload://4PZF5Suxy5Dnd91oapohhUmGZjh.png)
2

@zcp Please can you post the output of the command

cryosparcm rtpcli "get_session_doc('P99', 'S9')['exposure_groups']"

(replacing P99, S9 with the actual project and session IDs of the problematic CryoSPARC Live session).

3

really appreciate your quick response. I run this command as you mentioned. it gives messages like this below

cryosparcm rtpcli "get_session_doc('P38', 'S2')['exposure_groups']"
[{'defect_path': None, 'exp_group_id': 1, 'file_engine_enable': True, 'file_engine_filter': '*.tif', 'file_engine_interval': 10, 'file_engine_min_file_size': 0, 'file_engine_min_modified_time_delta': 0, 'file_engine_recursive': False, 'file_engine_strategy': 'entity', 'file_engine_watch_path_abs': '/data/zcp/cryosparc-live/movie', 'final': True, 'gainref_path': '/data/zcp/cryosparc-live/movie', 'ignore_exposures': False, 'num_exposures_found': 1755, 'num_exposures_ready': 0}]
4

The Gain Reference Path parameter of the Exposure Group in the Live session’s configuration

currently seems to point to a directory, but should point to the absolute path of a compatible file, like
/data/zcp/cryosparc-live/movie/gain_file.mrc (if movies are not already gain-corrected).

5

Thanks. I sure this movies are gain-corrected. below is setting. I do not think the path for movies is not incorrect

image
image1028×675 33.5 KB

6

what wtempel means is: the “directory to watch” can be a directory like /data/zcp/cryosparc-live/movie -but- the “gain reference path” must be filled out all the way to the exact single file ending in .mrc or .tif or similar like /data/zcp/cryosparc-live/movie/gain_file.mrc

7

what an amazing support team. really appreciate it. it works well after i tried as you mentioned(change gain path to /data/zcp/cryosparc-live/movie/gain.mrc ).