When attempting to import movies from EMPIAR-10500 (Falcon 4 data) with the provided gain reference, I get the attached error. Inspecting the header of the gain reference, it is 8192x8192. Is there a reason why EER data is restricted to 4096x4096 for the gain reference?
Also in the EMPIAR entry, it states:
“Please use gainImage_8k_defMasked.mrc as the gain reference without any transformation. Note that the EER gain reference has different convention from those for K2/K3 cameras. That is, the raw values must be divided, not multiplied, by the gain. RELION automatically handles this.”
Does cryosparc also automatically handle this difference in the application of gain references for EER data?
[CPU: 2.72 GB] Traceback (most recent call last): File "cryosparc_master/cryosparc_compute/run.py", line 84, in cryosparc_compute.run.main File "/home/exx/cryosparc/cryosparc_master/cryosparc_compute/jobs/imports/run.py", line 823, in run_import_movies_or_micrographs gshapey, gshapex = read_gain_reference(gainref_abs_path, shape_only=True, data_format=data_format) File "/home/exx/cryosparc/cryosparc_master/cryosparc_compute/jobs/imports/run.py", line 809, in read_gain_reference assert gshapex == gshapey == 4096, "Gain reference for EER must be 4096 x 4096" AssertionError: Gain reference for EER must be 4096 x 4096