Dear colleagues,
Could you please prompt is the following AFIS making sense and shall I count is as 9?
(K3)
Thank you.
Kind regards,
Dmitry
9 would be a good fit.
Depending on resolution achieved and total number of acquisitions you could split it further (i.e. 36) but it doesn’t usually make a lot of sense to do so.
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Thank you, @rbs_sci. The initial dataset is quite large—over 20,000 movies. So, if I understood correctly, a good strategy is starting with 9 and when I have the final particles set try 9 and 36. Thank you again.
Kind regards,
Dmitry
Depending how long the acquisition took, I’d split temporally (perhaps into three groups) rather than by individual shift positions unless you reach <1.4 Å or so… if the microscope has a cold-FEG, look up the flash timings and split at the flashes - that squeezed out a little extra for an apoferritin dataset I tested. Good luck! 
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wow 
Thank you! would you please indicate where this flashing timings can be observed (in which protocol - probably curation?)
On Thermo microscopes you need to look in the Health Monitor: I think you need Service Mode access. There’s no easier way that I know of; you need to manually check Emission Current in Health Monitor → GUN (CFEG) → Emission. You can see high-T and low-T flashes easily in the plot vs. time.
On JEOL 'scopes, I’m not sure, but since most JEOL users use SerialEM, should be possible to add something in the run script to write to a logfile when a flash is carried out…?
Then make a note of the times and split manually.
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thank you, @rbs_sci . I will try to check. It would be nice if such values could be tracked in the micrographs and corrected as a type of aberration in CryoSPARC (as a possible new feature?).