Duplicate particles emerging after re-extraction

Particle Picking
1

Hi everyone,

I have picked particles from my dataset using Topaz, adjusted the extraction radius to avoid the same particle being picked multiple times, and then also ran a remove duplicate particles job after the final extraction (the particles were binned 4X). At this point no duplicate particles were seen. However once I re-extracted the particles after filtering, one-fifth of the particles are now flagged as duplicates. My particles are close to each other, and I read in another thread that this may lead to alignments shifting and overlapping. Is there any way of avoiding this, or are these genuinely duplicates which I must remove from the dataset?

2

Likely genuine duplicates. The new extraction should print micrograph thumbnails with white circles, check for overlaps. Or use inspect picks. All tools for duplicate analysis are to help you get a single pick of each particle. Up to you to decide if it’s near neighbors or double picks

1 Like
3

Thanks, it didn’t occur to me to use inspect picks!