I am having a bit of a hard time figuring out how to properly switch between downsampled and original particles from the example provided.
-I have a set of extracted particles job: J90
Now I want to run a 3D helical refinement job using the J95 selected classes but with non-down-sampled pixel size. ii.e. how do i refer to the subset of non-down-sampled particles referenced by the selected down-sampled classes (J95)?
In the expanded inputs to your helical refinement job, first drag the input corresponding to the selected classes from J95. Click the drop down to expand it - you will see different slots, corresponding to e.g. the image (blob) and the CTF parameters. From the outputs of the extraction job (J93), drag the particles.blob slot and replace the existing one. This will replace the particle images with the non-downsampled versions, while keeping the selection the same.
But J93 is the down-sampling job, not the extraction job. The extraction job is J90. However, it seems that I am allowed to drag the blob entry from the down-sampling job (J93) but not from the extraction job J90.
see:
Trying to drag the extraction output blobs (J90) into the refinement job results in all all the input particle stack entries to be colored red so it seems that this is not allowed (??).
The refinement using the down-sampling job blobs (J93) goes through but it is using the down-sampled particles.
