Does re-extraction re-center particles?

Features and Functionality
#1

When I re-extract particles do the particles get re-centered, or are offsets discarded? Say I do a homogenous refinement, then use that as the input for an extraction job, are the new particles centered on the aligned coordiates, or on the original coordinates?

Thanks
RJ

#2

CryoSPARC does not recenter particles upon extraction. Apparently this will be an option in the next version

2 Likes
#3

Thought so. Thank you, sir!

#4

Hi all,

Re-centering on aligned shifts in Extract from Micrographs is added as an option in cryosparc v3.0, released today: cryosparc.com/updates. Thanks!

Best,
Michael

3 Likes