I have a question related to the import files from cryoSPARC to Relion3.
I have followed instruction from the page Export from cryoSPARC v2 and converted my parlicles.cs file to particles.star.
csparc2star.py my_file.cs my_file.star
I used an ab initio model (.mrc map) generated by cryoSPARC, as a reference and converted .star file as an input for the class3D job in Relion but it gives me an error message:
ERROR The reference pixel size is 2.108 A/px, but the pixel size of the first optics group of the data is 1.054 A/px!
The reference box size is 128 px, but the box size of the first optics group of the data is 256 px!
If I run Relion 3D classification job with a .mrc map generated from cryoSPARC refinement job instead everything is working fine without an error.
Could you please explain why this maps has different parameters (pixel size and box size)?
Or if they are the same, why in my Relion jobs it recognized differently?