Thank you for the quick response. I might not be understanding. For reference, the .mrc file I get from “ctf/diag_image_path” has entries that appear to range between -1 and 1, which suggests to me that it’s already log scaled. One thing I’ve tried is linearly normalizing the array to have values between 0 and 255 before using pyplot.imshow with vmin=0 and vmax=255, but that just gives me the result from my original post. Presumably, with those settings, it should be using all greyscale values, but I still don’t get anything like what’s displayed by cryosparc.