I’m mocking around with defocus refinement in my reconstruction of a small protein complex (~100kDa). The defocus range histogram is a bit wider than the one shown in the tutorial but I’m not so much worried about that. What strikes me is that the defocus error landscape looks very different from the tutorial, by far not such a beautiful local minimum.
Does anyone else get these error landscapes? Does this mean the defocus refine is bad? Is this because the refinement itself isn’t great (which it isn’t, probably sitting around 6-8A, it’s a challenging reconstruction)? I have additional tilt data from this particle which is why I’d really like to refine the per particle defocus…
These are the parameters I used. I tried using a different mininum fit res in other refinement jobs which din’t seem to help, I haven’t tried it on this exact one actually…
Any help is much appreciated!