We’re in the process of improving 3D classification in cryoSPARC. Initially, we are working on implementing 3D classification without alignment. As part of our internal testing, we’re looking for datasets where cryoSPARC’s heterogeneous refinement has not performed well, but where 3D classification without alignment has proven to be a useful step.
We would appreciate any pointers! Please feel free to also DM me if you’d like to (confidentially) share data.