CTF estimation error of v2




I just installed the v2 and I am running into the following error with the CTF estimation step. Is there anyone can help me out?

Traceback (most recent call last):
File “cryosparc2_worker/cryosparc2_compute/run.py”, line 78, in cryosparc2_compute.run.main
File “cryosparc2_compute/jobs/ctf/run.py”, line 60, in run_ctf_estimation
N_D = len(micrographs_dset)
TypeError: object of type ‘NoneType’ has no len()



Hi @Chen, it looks like you have not connected the micrographs to the CTF estimation job. Please clear the job, and then press “B” to start building it again, then drag and drop the micrographs you imported previously, into the CTF estimation job. See for example, section 1.7 here.


Hi @spunjani,

Thank you for your reply. It solved my problem.