Hi everyone,
I am fairly new to cryoEM and trying to convert particles that I have processed from cryoSPARCv4.2.1 to Relion5. I have done the data processing in cryoSPARC and would like to perform Bayesian polishing in Relion. I have followed Method 1 in the “cryosparc & Bayesian polishing” page of the github page for csparc2star.py. The entire workflow runs smoothly up until I run Bayesian polishing where I get the following error:
I looked into my .star file and I do have some columns (rlnAngleRot, rlnAngleTilt, rlnAnglePsi, and rlnRandomSubset) but I do not have (rlnOriginX and rlnOriginY). I am unsure why Relion cannot read some of the columns that I do have information for but I am also concerned why I do not have rlnOriginX and rlnOriginY. The columns I have are as follows:
If anyone can provide insight into how to approach my issue or if there is simple solution, I would really appreciate it! Thank you in advance and for your time!