Cryosparc to relion for 3d classification

Import and Export
1

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I want to export the an-initial model processed on cryosparc to relion for 3d classification, so I used the script csparc2star.py, but an error occurred when I used it. I would like to ask you why this is? Did I turn the data wrong?

Thank you！

2

Hi, it looks like you only used the passthrough_particles.star, while you need to also use the particles.star so the command should be something like, “csparc2star.py cryosparc_P130_J160_final_iteration_particles.cs P130_J160_passthrough_particles.cs from_csparc.star”

4

@user123 Thank you very much. The script run successfully.

5

No problem! Be sure to reference Dan Asarnow’s detailed guide on the use of pyem.