Creating templates from imported 3D volume using Chimera

Troubleshooting
4

  1. Generate a 3D map on scale with your data using ab initio followed by homogeneous refinement. The map can be junk. You need the dimensions, not the content. You can use a small subset of the particles to expedite process.

  2. Read map into chimera and move your PDB to center of this junk to generate centered templates

  3. Make a map of your PDB same way you did above

  4. Resample the map on the same grid dimensions as your data
    vop resample #1 onGrid #0
    Note: your map was read in first (#0). Your PDB was read in second (#1)

4 Likes