Hi,
I processed my micrographs using Topaz Denoise, but when I attempt manual particle picking in CryoSPARC, I encounter an error: “Could not get image for micrograph.” The micrograph information is present, but I am unable to open them for particle picking.
After investigating the issue, I discovered that the pixel value is mismatched between the input micrographs and the denoised output.
I would greatly appreciate any guidance on resolving this issue.
For reference, I have attached a screenshot of the manual particle picking error.
Rajnesh