Could not get image from topaz denoise in manual picking

Particle Picking
1

Hi,

I processed my micrographs using Topaz Denoise, but when I attempt manual particle picking in CryoSPARC, I encounter an error: “Could not get image for micrograph.” The micrograph information is present, but I am unable to open them for particle picking.

After investigating the issue, I discovered that the pixel value is mismatched between the input micrographs and the denoised output.

I would greatly appreciate any guidance on resolving this issue.

For reference, I have attached a screenshot of the manual particle picking error.

Screenshot from 2025-02-07 10-17-50
Screenshot from 2025-02-07 10-17-501124×913 193 KB

Rajnesh

2

Hi Rajnesh,

I am getting the same problem. May I ask if you solve the problem eventually?

Amy.

3

Hi @Amy @rkyadav0889 have you tried the CryoSPARC Micrograph Denoiser? We generally find it produces great denoising results

2 Likes
4

I don’t think this is directly Topaz related, but a bug in the Viewer which sometimes pops up even with CryoSPARC’s own denoiser. I’ve never worked out what specific sequence (Patch Motion/Patch CTF/Denoise/Curation or different order) is guaranteed to trigger it.

5

@rbs_sci Next time you encounter the error, please can you check under Metadata|Log for any errors.

1 Like
6

Will do! :slight_smile: Not seen it with 4.7.x so far.