Cant load tiff-s as movies

Import and Export
1

Hi,
we got our 2 “raw” data sets from TS, and on disc1 we have .tiff files, …_Fraction.tiff files and xml-s
on disc2 we have .mrc files, .xml-s and _Fraction.tiff-s.
When I try to import tiff-s like movies it runs to error, saying that

  File "cryosparc_master/cryosparc_compute/run.py", line 95, in cryosparc_master.cryosparc_compute.run.main
  File "/opt/cryosparc/cryosparc_worker/cryosparc_compute/jobs/imports/run.py", line 924, in run_import_movies_or_micrographs
    assert datashape[0] > 1, "Data file %s has only 1 frame - import as micrograph instead" % (abs_path)
AssertionError: Data file /rhome/PROTMOD/cryosparc/ELTE_data/20240220_095145_ELTE_DEMO_GRID01/Images-Disc1/GridSquare_19042588/Data/FoilHole_21159242_Data_20070855_0_20240221_102118.tiff has only 1 frame - import as micrograph instead

I cant import them as micrographs because that job accepts only mrc files.

Dataset nr.2:When i try to import the mrc files like movies it runs to error wtih this message:

  File "cryosparc_master/cryosparc_compute/run.py", line 95, in cryosparc_master.cryosparc_compute.run.main
  File "/opt/cryosparc/cryosparc_worker/cryosparc_compute/jobs/imports/run.py", line 924, in run_import_movies_or_micrographs
    assert datashape[0] > 1, "Data file %s has only 1 frame - import as micrograph instead" % (abs_path)
AssertionError: Data file /rhome/PROTMOD/cryosparc/ELTE_data/20240222_142632_live_demo_grid02/Images-Disc1/GridSquare_20881477/Data/FoilHole_21949864_Data_21912496_5_20240222_205438.mrc has only 1 frame - import as micrograph instead

Then I tried to import az micrographs, it seemed to work, but the Patch Motion correction went to error with message:

  File "cryosparc_master/cryosparc_compute/run.py", line 62, in cryosparc_master.cryosparc_compute.run.main
  File "/opt/cryosparc/cryosparc_worker/cryosparc_compute/jobs/runcommon.py", line 1117, in check_default_inputs
    assert False, 'Non-optional inputs from the following input groups and their slots are not connected: ' + missing_inputs + '. Please connect all required inputs.'
AssertionError: Non-optional inputs from the following input groups and their slots are not connected: movies.movie_blob. Please connect all required inputs.

What do I do wrong? Which files should I import?

2

The files your importing:

FoilHole_21159242_Data_20070855_0_20240221_102118.tiff
FoilHole_21949864_Data_21912496_5_20240222_205438.mrc

Don’t have _fractions.tiff or _fractions.mrc in their names and doesn’t appear to be movies, but simple micrographs. What is the size of the files? You mention fractions files - are you not able to import them as movies?

Since you managed to import them as micrographs, patch motion should not work since there are no frames to work on. If these are the only files you have, you can skip ahead to CTF estimation.

3

You may want to request a explanation of the various files, or file types, from the facility where the data were recorded. The facility may also recommend which files to import, and how, if you let them know your processing aims.
If you find yourself wanting to import single-frame tiff files, you may consider converting them to mrc format prior to import.