I am just started to use the local refinement job trying to improve the resolution of one of the domains of the protein.
But I don’t know what to do after obtaining the map generated by local refinement.
Can I combine it with the original one so that the map region of this domain can be replaced by the local refined map?
Or can I only use it separately in model building?
Then what about the model validation?
Should I do individual validation?
Sorry about these newbie questions.
Also, I am wondering is there any difference between the " Local Refinement" job and "NU Refinement with the mask covering the region to refine " ?
One of my local refinement (New) job completed and I found it a complete reconstruction of my protein rather just a sub-volume covered by the local mask.
So I don’t have combine the maps and can treat it as a full construction for the following model building and validation?