Can I use same Topaz model for different datasets?


Is it acceptable to use the same Topaz model to do Topaz extract for different datasets?
My datasets are the same protein with different substrates.

Yes you can. Why don’t you try it first and see if the result from the second dataset makes sense? I’d make sure to use consistent parameters in Topaz Extract e.g. downsampling factors, or rather, pixel sizes after downsampling & particle radii between the two datasets.